|
KMID : 0613820230330040357
|
|
Journal of Life Science
2023 Volume.33 No. 4 p.357 ~ p.362
|
|
|
Isolation of Agarivorans sp. JS-1 and Characterization of Its β-Agarase
|
|
Kim Jin-Sun
Lee Dong-Geun
Yeo Go-Wun
Park Min-Joo
Lee Sang-Hyeon
|
|
|
Abstract
|
|
|
|
This report looks at an agar-degrading marine bacterium and characterization of its agarase. Agar-degrading marine bacterium JS-1 was isolated with Marine agar 2216 media from seawater from the seashore of Sojuk-do, Changwon in Gyeongnam Province, Korea. The agar-degrading bacterium was named as Agarivorans sp. JS-1 by phylogenetic analysis based on 16S rRNA gene sequencing. The extracellular agarase was prepared from the culture media of Agarivorans sp. JS-1 and used for characterization. Relative activities at 20°C, 30°C, 35°C, 40°C, 45°C, 50°C, 55°C, and 60°C were 70%, 74%, 78%, 83%, 87%, 100%, 74%, and 66%, respectively. Relative activities at pH 5, 6, 7, and 8 were 91%, 100%, 90%, and 89%, respectively. Its extracellular agarase showed maximum activity (207 units/l) at pH 6.0 and 50°C in 20 mM Tris-HCl buffer. The residual activity after heat treatment at 20°C, 30°C, and 50°C for 30 minutes was 90%, 70%, and 50% or more, respectively. After a 2-hour heat treatment at 20°C, 30°C, 35°C, 40°C, and 45°C, the residual activity was 80%, 68%, 65%, 63%, and 57%, respectively. At 50°C and above, after heat treatment for 30 minutes, the residual activity was below 60%. Thin layer chromatography analysis suggested that Agarivorans sp. JS-1 produces extracellular β-agarases as they hydrolyze agarose to produce neoagarooligosaccharides such as neoagarohexaose (20.6%), neoagarotetraose (58.5%), and neoagarobiose (20.9%). Agarivorans sp. JS-1 and its thermotolerant β-agarase would be useful in the production of neoagarooligosaccharides, showing functional activity such as inhibition of bacterial growth and delay of starch degradation.
|
|
|
KEYWORD
|
|
|
Agarivorans sp. JS-1, β-agarase, marine bacterium, neoagarooligosaccharides, TLC
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|